Anti-Mannan-binding lectin (MBL, human)
Mouse monoclonal antibody
Cat.No. HYB 131-10
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars (1). It consits of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum (2)
MBL purified from human donor plasma and adsorbed onto aluminum hydroxide.
HYB 131-10 is specific for MBL from human serum or plasma
The epitope is thought to be on the carbohydrate recognition domain and differs from that of HYB 131-01 and HYB 131-11
HYB 131-10 reacts strongly with MBL. Strong reaction is seen in ELISA with MBL coated directly onto the microtiter well. In Western blotting, HYB 131-10 reacts with human MBL in both its oligomerized state and as a single protein chain of 26 kDa. HYB 131-10 can be used to measure MBL in plasma or serum but is not specific for oligomerized MBL when used in combination with itself, but not with other MBL antibodies.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/40,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.3 µg/mL together with human albumin 8.5 µg/mL. Users should determine the optimal dilutions for their own purposes.
1. Kawasaki N, Kawasaki T, Yamashina I (1983) Isolation and characterization of a mannan-binding protein from human serum. J Biochem (Tokyo) 94:937-947.
2. Steffensen R, Thiel S, Varming K, Jersild C, Jensenius JC (2000) Detection of structural gene mutations and promoter polymorphisms in the mannan-binding lectin (MBL) gene by polymerase chain reaction with sequence-specific primers. J Immunol Methods 241:33-42.
Downloads
HYB 131-10.pdf (21.51 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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Antibody MSDS
April 23 2008
