Anti Complement factor H (human, ß1H-globulin)
Mouse monoclonal antibody, biotinylated
Cat.No. HYB 268-01B
Preparation: Biotinylated
Content: 100 µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.13 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Factor H is a regulatory factor of the alternative complement pathway. Factor H is a glycoprotein consisting of a single polypeptide chain with molecular mass of 155 kDa. Human plasma concentration is app. 500 µg/ml (1,2).
Factor H isolated from human plasma adsorbed onto aluminum hydroxide gel
HYB 268-01 is specific for human factor H (b1H-globulin)
Not determined
HYB 268-01 reacts strongly with factor H. A strong reaction is seen in a sandwich ELISA in combination with a polyclonal antibody against factor H (eg. Abcam AB8842), when testing normal human serum. In Western blotting HYB 268-01 reacts with factor H.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/5000 | |
| Immunoblotting | |||
| Immunohistochemistry |
The dilution guideline for ELISA is based on sandwich ELISA in combination with a polyclonal antibody against the antigen. Users should determine the optimal dilutions for their own purposes.
1. Law SKA, Reid KBM (1988) Complement In: In Focus (Ed. Male D) IRL Press: Oxford.
2. Bendtzen K et al (2000) Basal og klinisk immunologi. FADL´s Forlag A/S, Copenhagen.
Downloads
HYB 268-01B.pdf (28.66 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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