Anti Complement factor H (human, ß1H-globulin), biotinylated
Mouse monoclonal antibody
Cat.No. GAU 020-03B
Preparation: Biotinylated
Content: 100 µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Factor H is a regulatory factor of the alternative pathway of complement activation. Factor H is a glycoprotein consisting of a single polypeptide chain with a molecular mass of 150 kDa.
Human Factor H (1).
GAU 020-03 is specific for complement factor H in human serum and plasma (1).
Epitope specificity differs from that of GAU 018-03 (1).
GAU 018-03 can be used as a capture antibody in sandwich ELISA with GAU 020-03 as detection antibody.
This antibody pair specifically detects full length 150 kDa factor H and does not cross react with the truncated forms of factor H (1).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:16,000 | 1,2 |
| Immunoblotting | Yes | ||
| Immunohistochemistry |
1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.
2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.
3. Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm. pharm 24:119-134.
Downloads
GAU 020-03B.pdf (23.49 kB) Product specification
Complement flyer.pdf (422.22 kB)
Complement flyer
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