BioPorto Diagnostic

AntibodyShop

Anti Complement factor B (caiman)

mouse monoclonal antibody

Cat.No. KSK 002-01

Subclass IgG2a/k

Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 無 and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8慢

Antigen:

Complement factor B plays an imortant role in the alternative complement activation pathway by complexing with C3b to create the active C3 convertase. The alternative activation pathway was the first to evolve, thus the components involved are very similar in physological different species. The caiman complement factor B is similar to the mammalian counterpart and consists of a single polypeptide chain of approximately 90 kDa which upon activation is cleaved in a Ba fragment (60 kDa) and a Bb fragment (30 kDa).

Immunogen:

Recombinant von Willebrandt domain of caiman factor B

Specificity:

KSK 002-01 reacts with unactivated caiman complement factor B and the Bb fragment of activated factor B.

Epitope specificity:

Reacts with the von Willebrand domain of caiman factor B

Reactivity:

KSK 002-01 is suitable for Western blotting, immunoprecipitation, and affinity purification on caiman factor B.

In ELISA KSK 002-01 reacts with native caiman factor Bb or intact factor B. Caiman plasma can inhibit binding of KSK 002-01 to a coat of the recombinant immunogen.

Application: