Anti Complement factor B (human)
Mouse monoclonal antibody
Cat.No. HAV 005-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Factor B is a component of the alternative complement pathway. The protein is a single chain polypeptide with a molecular mass of 93 kDa. Factor B is cleaved by factor D into the Ba component (30 kDa) and the Bb (63 kDa). Bb together with C3b forms the alternative pathway C3 convertase C3bBb. Plasma concentration is approximately 200 µg/ml (1).
Factor B isolated from human plasma
HAV 005-02 is specific for human factor B
Epitope specificity differs from that of HYB 008-06. Epitope specificity differs from that of HYB 008-02 but slightly overlap as determined by inhibition ELISA.
HAV 005-02 reacts strongly with factor B. Strong reaction is seen in ELISA with factor B coated directly onto the microtiter well, and also when tested in sandwich ELISA in combination with a polyclonal antibody against factor B (e.g. DAKO A 343). In Western blotting after SDS-PAGE, HAV 005-02 reacts with factor B in non-reduced form only.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:6500 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Kolb WP, Morrow PR, Tamerius JD (1989) Ba and Bb fragments of factor B activation: fragment production, biological activities, neoepitope expression and quantitation in clinical samples. Complement Inflamm 6:175-204.
Downloads
HAV 005-02.pdf (14.69 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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