Anti Complement component C9 (human)
Mouse monoclonal antibody
Cat.No. ABS 004-53
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Complement factor C9 is a 71-kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and have an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). Primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 µg/mL, and up regulated from hepatocytes by IFN-g.
Complement factor C9
ABS 004-53 is specific for complement factor C9
Not determined
ABS 004-02 recognizes C9 in human serum diluted 1:50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 µg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:20000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The Complement Facts Book. 2000. Bernard J. Morlay and Mark J. Walport (eds.). Academic Press, London.
Downloads
ABS 004-53.pdf (12.00 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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