Anti Complement component C4 (human)
mouse monoclonal antibody
Cat.No. HYB 162-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b. Together C2a and C4b forms the classical C3 convertase (1). The molecular mass of C4 is 204 kDa and it consists of 3 subunits: a (97kDa), b (75 kDa) and g (33 kDa).
C4 isolated from human plasma
HYB 162-04 has specificity for human C4
Epitope specificity differs from that of HYB 162-02, as determined by inhibition ELISA.
HYB 162-04 reacts strongly with C4. Strong reaction is seen with normal human serum, and no reaction is seen with serum depleted of C4, when tested in sandwich ELISA in combination with a polyclonal antibody against C4 (e.g. DAKO A065). HYB 162-04 also reacts with C4b deposited on coated antibody molecules. In Western blotting after SDS-PAGE, HYB 162-04 reacts with C4, in reduced as well as non-reduced forms.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:32,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
Complement flyer
1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
2. Petersen SV, Thiel S, Jensen L, Vorup-Jensen T, Koch C, Jensenius JC (2000) Control of the classical and the MBL pathway of complement activation. Mol Immunol 37:803-11.
