Anti Complement component C3 (human)
Mouse monoclonal antibody
Cat.No. HYB 005-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds (1,2).
C3 isolated from human plasma
HYB 005-01 is specific for human C3
Epitope specificity differs from that of HYB 030-08 and HAV 003-05 but slightly overlap as determined by inhibition ELISA..
HYB 005-01 reacts strongly with C3. Strong reaction is seen in ELISA with C3 directly coated onto the microtiter well, and also when tested in sandwich ELISA in combination with a polyclonal antibody against C3 (e.g. DAKO A 0062).
In Western blotting after SDS-PAGE, HYB 005-01 reacts with C3 in reduced as well as non-reduced forms.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:16,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
2. Morley BJ and Walport MJ (2000) The Complement FactsBook. Academic Press, London, UK.
Downloads
HYB 005-01.pdf (11.92 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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