Anti Complement component C3 (human), beta-chain genetic variant
Mouse monoclonal antibody
Cat.No. HAV 004-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of alpha and beta chains (110 kDa and 75 kDa respectively) held together by disulfide bonds (1,2).
C3 isolated from human plasm
HAV 004-01 is specific for an allotypic marker on human C3 (Leu instead of Pro in codon 314 of exon 9), which occurs regulsaly in the beta-chain of C3F and occasionally in the beta-chain of C3S (3,4). F and S mean fast and slow, respectively, on agarose gel electrophoresis. HAV 004-01 reacts with the 75-kDa beta-chain band on SDS-PAGE immunoblotting of reduced C3, and reacts with 20-kDa and 17-kDa beta-chain fragments produced by cyanogen bromide cleavage (5).
Epitope specificity differs from that of HAV 003-05
HAV 004-01 reacts strongly with C3. Strong reaction is seen in ELISA with C3 coated directly onto the microtiter well, and also when used as detection antibody in sandwich ELISA in wells coated with a polyclonal antibody against C3.
In Western blotting after SDS-PAGE, HAV 004-01 detects the above-mentioned allotypic marker on the beta-chain of C3, closely related but not completely specific to the fast-moving form of C3, C3F.
HAV 004-01 can be cleaved by papain to produce Fab fragments.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | |
| Immunoblotting | Yes | 3, 5 | |
| Immunohistochemistry | Not determined |
1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
2. Morley BJ and Walport MJ (2000) The Complement Facts Book. Academic Press, London, UK.
3. Koch C, Behrendt N (1986) A novel polymorphism of human complement component C3 detected by means of a monoclonal antibody. Immunogenetics 23:322-325.
4. Botto M, Fong KY, So AK, Koch C, Walport MJ (1990) Molecular basis of polymorphisms of human complement component C3. J Exp Med 172:1011-1017.
5. Behrendt N, Hansen OC, Ploug M, Barkholt V, Koch C (1987) Localization and functional significance of a polymorphic determinant in the third component of human complement. Mol Immunol 24:1097-1103.
Downloads
HAV 004-01.pdf (12.59 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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April 23 2008
