Anti Complement component C2 (human)
mouse monoclonal antibody
Cat.No. HYB 050-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102 kDa (2).
Complement component C2 isolated from human plasma (1)
HYB 050-04 is specific for C2 from human plasma/serum. Crossreactivity to C2 from other species has not been tested.
Epitope specificity differs from that of HYB 050-05 and HYB 050-08 as determined by inhibition ELISA.
HYB 050-04 reacts with human C2 in both native and denatured forms. In Western blotting after SDS-PAGE, HYB 050-04 reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions. In ELISA, strong reaction is seen with C2 directly coated onto the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:20,000 | 1 |
| Immunoblotting | Yes | 1 | |
| Immunohistochemistry | Not determined |
1. Stenbaek EI, Koch C, Barkholt V, Welinder KG (1986) Human complement component C2: production and characterization of polyclonal and monoclonal antibodies against C2. Mol Immunol 23:879-886.
2. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
Downloads
HYB 050-04.pdf (13.38 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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