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Anti Surfactant protein D (human, hSP-D)

Mouse monoclonal antibody

Cat.No. HYB 245-02

Subclass IgG1/k

Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC

Antigen:

Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals (2).

Immunogen:

Recombinant neck CRD SP-D

Specificity:

HYB 245-02 is specific for human SP-D

Epitope specificity:

Not determined

Reactivity:

In Western blotting after SDS-PAGE HYB 245-02 reacts strongly with SP-D, both in reduced and non-reduced forms. HYB 245-02 only reacts with SP-D in the presence of Ca++.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes	1:1000
Immunoblotting	Yes	1:3000
Immunohistochemistry	Yes

Application Remarks:

References:

1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.
2. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of gp-340, a putative opsonin receptor for lung surfacant protein D. Proc Natl Acad Sci USA 96:10794-9.
3. Madsen J, Kliem A, Tornoe I, Skjodt K, Koch C, Holmskov U (2000) Localization of lung surfactant protein D on mucosal surfaces in human tissues. J Immunol 164:5866–70.
4. Johansson J, Curstedt T (1997) Molecular structures and interactions of pulmonary surfactant components. Eur J Biochem 244:675-693.