Anti Surfactant protein D (human, hSP-D)
mouse monoclonal antibody
Cat.No. HYB 245-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals (2).
Recombinant neck CRD SP-D
HYB 245-01 is specific for human SP-D
Not determined
In immunohistochemical staining on paraffin embedded tissues, HYB 245-01 reacts specifically with SP-D. Immunoreactivity can be found in epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder.
In Western blotting after SDS-PAGE HYB 245-01 reacts strongly with SP-D, both in reduced and non-reduced forms.
HYB 245-01 can be used for ELISA with Ca++ present in the buffer.
| Method | Usability | Dilution guideline | References |
| ELISA | No | 1:250 | |
| Immunoblotting | Yes | 1:20,000 | 3, 6 |
| Immunohistochemistry | Yes | 1:800 | 2, 3, 6 |
1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.
2. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of gp-340, a putative opsonin receptor for lung surfacant protein D. Proc Natl Acad Sci USA 96:10794-9.
3. Madsen J, Kliem A, Tornoe I, Skjodt K, Koch C, Holmskov U (2000) Localization of lung surfactant protein D on mucosal surfaces in human tissues. J Immunol 164:5866–70.
4. Johansson J, Curstedt T (1997) Molecular structures and interactions of pulmonary surfactant components. Eur J Biochem 244:675-693.
5. Kang W, Nielsen O, Fenger C, Madsen J, Hansen S, Tornoe I, Eggleton P, Reid KB, Holmskov U (2002) The scavenger receptor, cysteine-rich domain-containing molecule gp-340 is differentially regulated in epithelial cell lines by phorbol ester. Clin Exp Immunol 130:449-458.
6. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.
