Anti von Willebrand factor (human, vWf)
mouse monoclonal antibody
Cat.No. HYB 060-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Von Willebrand factor (vWf) is a series of self-aggregated structures, all derived from common glycoprotein subunits, synthesized in endothelial cells and megakaryocytes. The molecular mass of the monomeric vWf unit is 225 kDa. vWf binds to circulating factor VIIIc (antihemophilic factor), hereby stabilizing the latter thus beeing an important factor in the normal primary haemostasis (1).
Factor VIII complex isolated from human plasma
HYB 060-02 has specificity for human vWf from human serum or plasma
Epitope specificity differs from that of HYB 060-01
HYB 060-02 reacts strongly with vWf. Strong reaction is seen in ELISA with vWf directly coated onto the microtiterplate, and also when used in sandwich ELISA in combination with a polyclonal antibody against the antigen (e.g. DAKO A082).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:40000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
