Anti-Thrombin (human)
Mouse monoclonal antibody
Cat.No. HYB 109-04
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide Storage: In the dark at 4-8ºC
Thrombin is the active enzyme that splits fibrinogen into fibrin in final stage of blood coagulation. It is generated from its circulating inactive precursor, prothrombin, by the action of factor Xa aided by factor Va, phospholipid and calcium ions. Thrombin is a glycoprotein formed by a 6-kDa A chain linked by a disulfide bridge to a 31-kDa B chain which carries the serine protease catalytic site
Thrombin isolated from activated human plasma and adsorbed onto aluminum hydroxide gel
HYB 109-04 binds to human thrombin and to complex of human thrombin with the thrombin inhibitor hirudin. No cross-reaction is seen with prothrombin. Cross-reactivity with thrombin from other species has not been tested.
Not determined
HYB 109-04 reacts strongly in ELISA with thrombin coated directly onto the microtiter wells. Biotinylation of thrombin blocks antibody binding.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/500 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at high concentration. Users should determine the optimal dilutions for their own purposes.
