Anti-D-dimer (human)
Mouse monoclonal antibody
Cat.No. ABS 015-22
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
D-dimer is a fibrin degradation product, a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis.
Purified human D-dimer adsorbed onto aluminum hydroxide gel.
ABS 015-22 binds human D-dimer. Cross-reacts <2% with directly coated D-monomer but not with directly coated fibrinogen.
When tested in a pair with itself, with ABS 015-28 and with a fibrinogen-specific polyclonal antibody, the epitope appears to relate to the dimenzation site of D-dimer.
ABS 015-22 binds free D-dimer in solution.
ABS 015-22 works as capture antibody in sandwich ELISA with biotinylated ABS 015-22 or ABS 015-28 as detection antibody.
Biotinylated ABS 015-22 works as detection antibody in sandwich ELISA with itself (ABS 015-22) or ABS 015-28 as capture antibody. The combination with ABS 015-28 as capture antibody is recommended.
| Method | Usability | Dilution guideline | References |
| ELISA | YES | 1/8000 | |
| Immunoblotting | Not determined | ||
| Immunoblotting | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for D-dimer coated at 0.3 µg/mL. Users should determine the optimal dilutions for their own purposes.
