Anti Antithrombin III (human, denatured)
Mouse monoclonal antibody
Cat.No. HYB 228-12
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.
Antithrombin III isolated from human plasma (1)
HYB 228-12 is specific for human AT III, denatured
Epitope specificity differs from that of HYB 230-01, HYB 228-08 and HYB 228-10.
HYB 228-12 reacts strongly with denatured AT III. Strong reaction is seen in ELISA with denatured AT III coated directly onto the microtiter well. HYB 228-12 reacts with AT III in both reduced and non-reduced forms in Western blotting after SDS-PAGE.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:25000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Sorensen PJ, Ezban M, Jessen TE, Sas G, Blasko G, Jorgensen M (1990) Purification and characterisation of the pathological antithrombin III "Aalborg". Thromb Res 57:807-812.
