Anti Antithrombin III (human, native and denatured)
Mouse monoclonal antibody
Cat.No. HYB 228-08
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.
Antithrombin III isolated from human plasma (1)
HYB 228-08 i specific for human AT III, native and denatured
Epitope specificity differs from that of HYB 230-03, HYB 228-10 and HYB 228-12.
HYB 228-08 reacts strongly with AT III both native and denatured forms. Strong reaction is seen in ELISA with both native and denatured AT III directly coated onto the microtiter well and also when used as detection antibody in sandwich ELISA in combination with a polyclonal capture antibody (eg. DAKO A 296).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:16,000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Sorensen PJ, Ezban M, Jessen TE, Sas G, Blasko G, Jorgensen M (1990) Purification and characterisation of the pathological antithrombin III "Aalborg". Thromb Res 57:807-812.
