Anti Vitronectin (human)

Mouse monoclonal antibody

Cat.No. CSI 003-08

Subclass IgG1/k
Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC

Antigen:

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin (2).

Immunogen:

Human vitronectin purified from plasma by heparin-affinity chromatography

Specificity:

CSI 003-08 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
CSI 003-08 cross-reacts with vitronectin from dog, to a lesser extent with cat and sheep.

Epitope specificity:

Epitope is located within a cyanogens bromide cleavage fragment comprising aa 1-310

Reactivity:

CSI 003-08 binds nearly as well to native vitronectin as to denatured. It can be used to quantitate vitronectin in human plasma or serum in a sandwich ELISA with antibody CSI 003-02. CSI 003-08 inhibits the binding of PAI-1 to vitronectin. CSI 003-08 also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well.

Application:
Method Usability Dilution guideline References
ELISA Yes 1:30000 1, 2
Immunoblotting Yes 1:50
IHC Not determined
Application Remarks:
 
References:

1. Morris CA, Underwood PA, Bean PA, Sheehan M, Charlesworth JA (1994) Relative topography of biologically active domains of human vitronectin. Evidence from monoclonal antibody epitope and denaturation studies. J Biol Chem 269:23845-23852.
2. Underwood PA, Kirkpatrick A, Mitchell SM (2002) New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J 365:57-67.

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