Anti Perlecan (bovine)
Mouse monoclonal antibody
Cat.No. CSI 001-71
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability charateristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (2).
Bovine corneal endothelial cells
CSI 001-71 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
CSI 001-71 cross-reacts with human perlecan. Other species have not been tested.
Epitope is located in domain I
CSI 001-71 can be used in immunoprecipitation, ELISA and immunostaining of frozen PLP-fixed sections of bovine and human tissues. CSI 001-71 can be used in affinity chromatography to separate recombinant domain I from tissue culture fluid.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | 1 |
| Immunoblotting | Yes | 1:100 | 1 |
| Immunohistochemistry | Yes | 1:100 |
1. Whitelock JM, Murdoch AD, Iozzo RV, Underwood PA (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086.
2. Whitelock JM, Graham LD, Melrose J, Murdoch AD, Iozzo R, Underwood PA (1999) Human perlecan immunopurified from different endothelial cell sources has different adhesive properties for vascular cells. Matrix Biol 18:163-178.
3. Olsen BR (1999) Life without perlecan has its problems. J Cell Biol 147:909-912.
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CSI 001-71.pdf (22.86 kB) Product specification
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April 23 2008
