Anti Fibronectin (bovine)

Mouse monoclonal antibody

Cat.No. CSI 005-35

Subclass IgG1/k
Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC

Antigen:

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).

Immunogen:

Lysed bovine corneal endothelial cells and extracellular matrix

Specificity:

CSI 005-35 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue protein (vitronectin, elastin, collagen, laminin).
CSI 005-35 cross-reacts with human fibronectin. Other species have not been tested.

Epitope specificity:

Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of CSI 005-32

Reactivity:

CSI 005-35 can be used in ELISA, Western blotting, immunoprecipitation and immunostaining of frozen PLP-fixed sections of bovine and human tissues. The antibody can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody CSI 005-32.

Application:
Method Usability Dilution guideline References
ELISA yes 1:4000 1, 2, 3
Immunoblotting yes 1:100 1
Immunohistochemistry yes
Application Remarks:
 
References:
  1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin bindingand cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.
  2. Underwood PA, Steele JG, Dalton BA (1993) Effects of polystyrene surface chemistry on biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies. J Cell Sci 104:793-803.
  3. Di Girolamo N, Underwood PA, McCluskey PJ, Wakefield D (1993) Functional activity of plasma fibronectin in patients with Diabetes mellitis. Diabetes 42:1606-1613.

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