Anti-Type I Collagen
Mouse monoclonal antibody
Cat.No. CSI 008-01
Preparation: Protein-A purified
Content: Available in 200 ”L and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8șC
Type I collagen is present in connective tissue and bone where it makes up about 90% of the organix matrix. Defects in one or both alleles of the type I collagen gene causes brittle bone disease" (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in fragile bones.
Pepsin-solubilized, salt-fractioned type I collagen from human placenta (1)
CSI 008-01 is highly specific for native type I collagen and shows no evidence of cross-reactivity with type II, III, V and VI collagens in inhibition ELISA and immunoblotting, and no evidence of cross-reactivity with other connnective tissue proteins (laminin, fibronectin, elastin). CSI 008-01 reacts with human, calf, sheep, pig, dog and goat type I collagens as demonstrated by ELISA and immunoblotting, but does not cross-react with mouse, guinea pig, cat, rabbit, squirrel, horse, kangaroo or chicken type I collagen. CSI 008-01 has a Ka of approximately 2.5x106 M-1 for human type I collagen.
Epitope is located near the C-terminal of the molecule
CSI 008-01 can be used to detect collagens by ELISA. In immunoblotting CSI 008-01 detects human type I collagen only in its native triple helix form. CSI 008-01 can be used for immunohistochemistry on frozen unfixed sections of human (1,4), and bovine (2,3) skin. If fixation of tissue is required, acetone or ethanol is recommended.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/16,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Yes | 1, 2, 3, 4 |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 4 ”g/mL. Users should determine the optimal dilutions for their own purposes.
1. Werkmeister JA, Ramshaw JAM, Ellender G (1990) Characterisation of monoclonal antibody against native human type I collagen. Eur J Biochem 187:4369-443.
2. Werkmeister JA, Ramshaw JAM (1988) The use of immunohistology in studies on connective tissue organisation in hides and skins. Das Leder 39:145-151.
3. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.
4. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.
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April 23 2008
